ELISA Kits and DuoSet ELISA Development Kits
ELISA Kits are the foundation of most immunoassay systems. They allow researchers to measure antibodies, antigens, hormones, and other analytes in biological samples with accurate counting capabilities and a wide range of detection options. Their exacting analysis helps to understand biological systems, monitor diseases, and develop treatments. The quantification of biomolecules also provides quality control in industries like custom protein, antibody, and gene production. This link bosterbio.com
Unlike other testing methods that take days or weeks to process, ELISA kits are ready for immediate use and can be read in minutes. This allows healthcare professionals to act quickly for quicker, more accurate results. The sensitivity of ELISAs finds even tiny bits of specific substances, which can help to catch diseases early on and improve patient outcomes.
Custom Monoclonal Antibodies: The Future of Targeted Research
To perform ELISAs, the sample and a primary antibody are incubated together in an antigen-coated well. A secondary antibody that has been labeled with an enzyme, such as horseradish peroxidase (HRP) or alkaline phosphatase, is then added. The enzyme reacts with the sample and generates a chromogenic or fluorescent signal, which is detected with a spectrophotometer, fluorometer, or luminometer.
ELISAs are also available in competitive or sandwich formats and can be designed for many different targets, utilizing a wide variety of conjugates with HRP or AP. The development of ELISAs can be accelerated with DuoSet ELISA Development Kits that provide matched antibody pairs, standards, and streptavidin-HRP for optimal assay development. These kits are optimized for lot-to-lot consistency and assay performance, minimizing matrix effects and false positives.